Gene description for FCSK
Gene name	fucose kinase
Gene symbol	FCSK
Other names/aliases	1110046B12Rik CDGF2 FUK
Species	Homo sapiens
Experiment description of studies that identified FCSK in Colorectal cancer cells
1 Experiment ID 303 Identified molecule mrna Extracellular vesicle type Microvesicles MISEV EVs Identification method Microarray [Illumina] PubMed ID 19930720    Organism Homo sapiens Experiment description Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells Authors Hong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS Journal name BMC Genomics Publication year 2009 Sample Colorectal cancer cells Sample name SW480 Isolation/purification methods Differential centrifugation Ultrafiltration OptiPrep density gradient Flotation density 1.09 g/mL Molecules identified in the study Protein mRNA Methods used in the study Western blotting RT-PCR Microarray [Illumina] EV-TRACK -
2 Experiment ID 487 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name EpCAM affinity purified exosomes-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
3 Experiment ID 488 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23230278    Organism Homo sapiens Experiment description Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Authors Tauro BJ, Greening DW, Mathias RA, Mathivanan S, Ji H, Simpson RJ. Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Shed vesicles-Colorectal cancer cells (LIM1863) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130020: EV-METRIC:38%
4 Experiment ID 3338 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing EV-TRACK -
5 Experiment ID 3339 Identified molecule protein Extracellular vesicle type Exomeres MISEV EV-like nanoparticles Identification method Mass spectrometry PubMed ID 34887515    Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry ELISA RNA sequencing EV-TRACK -